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Sybe Safe as a cell marker

Sybe Safe as a cell marker - Protocols and Methods Forum

Sybe Safe as a cell marker - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 07-08-2009, 09:24 PM
Scott Brown
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Default Sybe Safe as a cell marker



Hi all,

This may seem a little random, i know PI can be used with flow, i was wondering if sybr safe or ethidium bromide can be utilised in the same way. The biology is the same, both etbr and sybr safe intercollate withing the DNA, so any advice or theoretical ideas would be much appreciated, or is this idea plagued with errors?

S

Scott Brown
PhD Candidate
Molecular Carcinogenesis Program
Children's Cancer Institute of Australia for Medical Research
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Children's Cancer Institute Australia for Medical Research is the only independent medical research institute in Australia devoted to research into the causes, prevention, better treatment and ultimately a cure of childhood cancer. Our vision is to save the lives of all children with cancer and to eliminate their suffering.

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Old 07-08-2009, 10:56 PM
DK
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Default Sybe Safe as a cell marker

In article <[Only registered users see links. ].net> , "Scott Brown" <[Only registered users see links. ].edu.au> wrote:

No, EB cannot be used in flow cytometry to distinguish dead cells.
Unlike propidium iodide, it is somewhat membrane permeable.

SYBR Safe is membrane permeable, too.

DK
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