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YNB plates not setting
I have a problem with getting my plates to set. In 1L the media contains ca. 700mg of various amino acids, 6,9g of Yeast Nitrogen Base, 2% Agar and sterile water. After autoclaving I add 20% sterile Glucose.
The first time I had problems getting them to set I started pHing them to around 7.5
That seemed to work, but now all of a sudden they're not setting anymore.
I haven't changed any of the ingredients.
Does anyone know any tips?
|media , plates , setting , yeast , yeast nitrogen base , ynb|
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