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#1
07-06-2009, 11:55 AM
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 YNB plates not setting Hi, I have a problem with getting my plates to set. In 1L the media contains ca. 700mg of various amino acids, 6,9g of Yeast Nitrogen Base, 2% Agar and sterile water. After autoclaving I add 20% sterile Glucose. The first time I had problems getting them to set I started pHing them to around 7.5 That seemed to work, but now all of a sudden they're not setting anymore.  I haven't changed any of the ingredients. Does anyone know any tips? Thanks  |
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| media , plates , setting , yeast , yeast nitrogen base , ynb |
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