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YNB plates not setting

YNB plates not setting - Protocols and Methods Forum

YNB plates not setting - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 07-06-2009, 11:55 AM
Pipette Filler
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Default YNB plates not setting



Hi,

I have a problem with getting my plates to set. In 1L the media contains ca. 700mg of various amino acids, 6,9g of Yeast Nitrogen Base, 2% Agar and sterile water. After autoclaving I add 20% sterile Glucose.

The first time I had problems getting them to set I started pHing them to around 7.5
That seemed to work, but now all of a sudden they're not setting anymore.
I haven't changed any of the ingredients.

Does anyone know any tips?

Thanks
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media , plates , setting , yeast , yeast nitrogen base , ynb


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