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Methods Digest, Vol 49, Issue 12

Methods Digest, Vol 49, Issue 12 - Protocols and Methods Forum

Methods Digest, Vol 49, Issue 12 - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 06-14-2009, 11:28 PM
TieQiao Wu
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Default Methods Digest, Vol 49, Issue 12



Hi,

Did you use prestained protein marker? You should see the marker on your
membrane very clearly using your transferring voltage and time setting.
What I can suggest at the moment is that you check your transfer buffer
and your sample buffer.

Cheers

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Today's Topics:

1. Western Blotting problems (Alsagaby, Suliman)


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Message: 1
Date: Sat, 13 Jun 2009 19:16:37 +0100
From: "Alsagaby, Suliman" <[Only registered users see links. ].uk>
Subject: Western Blotting problems
To: <[Only registered users see links. ].indiana.edu>
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Hello

I did western blot twice and every time I have the same problem in which
the PVDF membrane does not contain any protein although it contains
protein marker. The bands were visualized by staining the gel. So it
seems to me that the bands did not transfer to the membrane. I verified
the transfer duration: one for 12h at 30V and the other one was for 1h
at 100V.

I am great full if any body can help me and direct me to the right
protocol if I am doing wrong one.



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