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ER Special Dyes

ER Special Dyes - Protocols and Methods Forum

ER Special Dyes - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 06-10-2009, 10:06 AM
Jinxiang Wang
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Question ER Special Dyes



Dear Colleaguses,

I am going to localize a protein which is from soybean.Bioinformatic
analysis indicates it is maybe licalized in ER membrane.Initially I
would likt to fuse my protein to GFP and check its
localization.Alternatively I can employe antibody against my protein to
verify its localization. Certainly the later technique is
time-consuming. Would you kindly give me your suggestions related to
special marker dyes for ER or ER-special marker proteins? In addtion I
know some special dyes and antibody for ER-special marker proteins are
commercially availabe. I look foward to comments or ideas of experts to
guide me to select better dyes or marker protein Ab? Many thanks in advance.

Best regards.

Jinxiang


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----------------------------------------------------
Jinxiang Wang Ph.D

College of Resources and Environment Science
Root Biology Center
South China Agricultural University
Guangzhou 510642, P.R.China

Email: [Only registered users see links. ]
Tel: +86-20-85280156 (O)
Fax: +86-20-85281829


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  #2  
Old 06-10-2009, 07:17 PM
Cathal Garvey
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Default ER special dyes

I have it on good authority from a labmate that sucrose-gradients are
a great way to check for protein localisation. I gather it involves a
long centrifuge cycle but yields very reliable results.

On 10 Jun 2009, at 11:06, Jinxiang Wang <[Only registered users see links. ].cn> wrote:


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  #3  
Old 06-12-2009, 11:14 AM
Jinxiang Wang
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Default ER special dyes


--
----------------------------------------------------
Jinxiang Wang Ph.D

College of Resources and Environment Science
Root Biology Center
South China Agricultural University
Guangzhou 510642, P.R.China

Email: [Only registered users see links. ]
Tel: +86-20-85280156 (O)
Fax: +86-20-85281829


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  #4  
Old 06-12-2009, 04:37 PM
Dr Engelbert Buxbaum
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Default ER special dyes

Am 10.06.2009, 06:06 Uhr, schrieb Jinxiang Wang <[Only registered users see links. ].cn>:


check Molecular Probes (Invitrogen.com) for ER-specicic life-cell markers.
Alternatively of course you can use an fluorescent antibody against an
ER-specific protein, however, then the cell has to be permeabilised.
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