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I have a spectrophotometer (with cuvette). I want to measure total protein using BioRad Dye (BradFord method). How much protein (insect homogenate)and how much dye?
Is it 100 uL Protein + 1900 uL (1X) Dye? @ 595 nm
Please advise. Many thanks.
Mohammad Ali Al-Deeb
In article <email@example.com> , M Aldeeb <firstname.lastname@example.org> wrote:
Do a calibration curve with a known amounts of BSA (or whatever other
protein). Note the amount of absorbance where the curve deviates
sigificantly from linearity. Add enough of your lysate to get approximately
the same or lower intensity of blue color. And then add twice less lysate.
From the calibration curve and your two (or more) experimental points,
you can estimate total protein in your lysate.
|protein , total|
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