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#1
06-09-2009, 10:12 AM
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 Total Protein Dear All, I have a spectrophotometer (with cuvette). I want to measure total protein using BioRad Dye (BradFord method). How much protein (insect homogenate)and how much dye? Is it 100 uL Protein + 1900 uL (1X) Dye? @ 595 nm Please advise. Many thanks. Mohammad Ali Al-Deeb  |
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#2
06-09-2009, 09:24 PM
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| Total Protein In article <mailman.368.1244572475.21502.methods@net.bio.net> , M Aldeeb <moh_aldeeb@yahoo.com> wrote: Do a calibration curve with a known amounts of BSA (or whatever other protein). Note the amount of absorbance where the curve deviates sigificantly from linearity. Add enough of your lysate to get approximately the same or lower intensity of blue color. And then add twice less lysate. From the calibration curve and your two (or more) experimental points, you can estimate total protein in your lysate. DK |
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