Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protocols and Methods Forum
Register Search Today's Posts Mark Forums Read

Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


EDTA solution becomes slurry

EDTA solution becomes slurry - Protocols and Methods Forum

EDTA solution becomes slurry - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 05-31-2009, 12:29 PM
Yoram Gerchman
Guest
 
Posts: n/a
Default EDTA solution becomes slurry




EDTA will not dissolve until pH 8 is reached, hence the "white slurry". Simply
keep adding NaOH while measuring pH (I suggest using a pH paper, not an
electrode). When you get near pH=8 you will see sudden clearance of the
solution. You can definitely start with NaOH pellet (add a little and let it
dissolve completely before adding more) and use NaOH solution only for final
adjustment. Preparing EDTA solution is a slow process...
Yoram





------------------------------------------------------------------------
This message was sent using IMP, the Webmail Program of Haifa University

Reply With Quote
  #2  
Old 05-31-2009, 09:41 PM
Tom Knight
Guest
 
Posts: n/a
Default EDTA solution becomes slurry

Yoram Gerchman <[Only registered users see links. ].il> writes:

To save some time and frustration, you need about equimolar amounts of
NaOH and EDTA to dissolve the EDTA. Effectively, you are making
trisodium EDTA from disodium EDTA. You can add almost enough pelleted
NaOH to reach this point, then titrate with pH paper and 5M NaOH
solution to reach pH 8.0. Just a reminder, you cannot make a 1 M
solution -- the stock solution is 500 mM.

Reply With Quote
  #3  
Old 07-28-2009, 03:31 PM
briancady413
Guest
 
Posts: n/a
Default EDTA solution becomes slurry

On May 31, 5:41*pm, Tom Knight <[Only registered users see links. ].edu> wrote:

Tom, is there a reference for this? A colleague is attempting to make
1M EDTA, with great difficulty.
I guess I will search for solubility info.

Thanks in advance,

Brian Cady

Reply With Quote
Reply

Tags
edta , slurry , solution


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
EDTA solution becomes slurry Gourvendu Saxena Protocols and Methods Forum 0 05-28-2009 01:59 PM
Production of Monoclonal Antibodies - Technique in Mouse Cellbiogal Antibody Forum 2 01-08-2009 10:52 PM
Cylindrical wave, wave equation, and mistakes h_v_ansari@yahoo.com Physics Forum 2 10-26-2006 01:47 AM
préparation d'une solution EDTA dissodique 0.1M REY Forum De Chimie 3 09-26-2004 11:13 AM
Sci.chem FAQ - Part 6 of 7 Bruce Hamilton Chemistry Forum 0 01-15-2004 09:12 AM


All times are GMT. The time now is 06:11 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.13475 seconds with 16 queries