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Glyco stain

Glyco stain - Protocols and Methods Forum

Glyco stain - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 05-20-2009, 09:59 PM
SIVAKUMAR
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Default Glyco stain



Hi
Greetings
I have been working on archael protein. Does any one could help me
to figure out the problem in glycosylation staining with Proq
emerald 300. I am getting non specific staining that means it
stains even nonglycosylated protein which was run as negetive
control on SDS-Page 14% gel. Even with the candy cane marker,
nonglycosylated protein gives signal. Could any share your
experineses.
Thans, Siva


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  #2  
Old 05-22-2009, 06:06 PM
Dr Engelbert Buxbaum
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Default Glyco stain

Am 20.05.2009, 17:59 Uhr, schrieb SIVAKUMAR <[Only registered users see links. ]>:


Have you tried the classical PAS-staining, just as a sanity check? Are you
satining in the gels directly or on blots?
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