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#1
05-20-2009, 09:59 PM
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 Glyco stain Hi Greetings I have been working on archael protein. Does any one could help me to figure out the problem in glycosylation staining with Proq emerald 300. I am getting non specific staining that means it stains even nonglycosylated protein which was run as negetive control on SDS-Page 14% gel. Even with the candy cane marker, nonglycosylated protein gives signal. Could any share your experineses. Thans, Siva  |
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#2
05-22-2009, 06:06 PM
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| Glyco stain Am 20.05.2009, 17:59 Uhr, schrieb SIVAKUMAR <[Only registered users see links. ]>: Have you tried the classical PAS-staining, just as a sanity check? Are you satining in the gels directly or on blots? |
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