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#1
05-18-2009, 04:54 PM
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 RNA based FISH on frozen tissue samples Hello everyone, I was wondering if anyone had a protocol, or could point me in the direction of one, for fluorescent in situ hybridization (FISH) of RNA probes to gene transcripts on frozen tissue sections. I've found numerous references to this effect, but none that seem to precisely match what we need (we are looking for transcripts in muscle cells). If there were any other resources you folks could suggest, or advice and procedures from your own experiences, it would be most appreciated. -- Nicholas S. Geraci, MS Research Associate Molecular & Cellular Pathobiology Children's Memorial Research Center 2430 North Halsted Street Chicago, Illinois 60614 (773) 755-6399 lab (513) 505-5339 mobile  |
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| based , fish , frozen , rna , samples , tissue |
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