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RNA labeling at 5'

RNA labeling at 5' - Protocols and Methods Forum

RNA labeling at 5' - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 05-17-2009, 08:41 PM
nada mohamed
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Default RNA labeling at 5'



Dear all,
*
I am a PhD student and I am studying RNA-Protein interaction. I need a protocol to label my RNA at 5'end with biotin, I found some researches using megascript kit to incorporate biotin and others using Maxiscript I am confused which one is suitable ? and is there any alternative methods, also what is the criteria that must be taken in account for RNA biotin labeling (to besure that my RNA is not affected by biotin incorporation),*I mean the size of RNA the amount of*labeled*nucleotides. Any help will be appreciated.*



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