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Acid phosphatase

Acid phosphatase - Protocols and Methods Forum

Acid phosphatase - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 05-15-2009, 02:39 AM
Sakkthi subramaniyam
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Default Acid phosphatase



Hai can any one give me complete procedure for estimation of ACID
PHOSPHATASE, INVERTASE AND SUCROSE SYNTHASE with calculation formula.
Please dude I got procedures but I couldn't sort out how to arrive
mathematically.Here are references for those estimations

ACID PHOSPHATASE, Parfsh, R.W. (1974). J. Histochem. Cytochem., 9 : 542.

INVERTASE Sridhar, R. and S.H. Ou. (1972). Phillippine Phytopath., 8, 52-56.

SUCROSE SYNTHASE Wardlaw, I.F. and J. Willenbrink (1994). Aust. J.
Plant Physiol, 21,255-271.
--
With regards,
Sakkthi subramaniyam,
II. M.Sc.(Ag)

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  #2  
Old 05-15-2009, 04:49 PM
Dr Engelbert Buxbaum
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Default Acid phosphatase

Am 14.05.2009, 22:39 Uhr, schrieb Sakkthi subramaniyam
<[Only registered users see links. ]>:


Then you need to get a good book on enzymology and learn how to work this
out, it is not rocket science.

For example, if you release nitrophenol from p-nitrophenylphosphate by a
phosphatase, the determination is by photometry. The absorbance is related
to the concentration of NP (the product) by Lambert-Beer's law.
Concentration times volume gives amount, amount by time gives activity.
Activity divided by protein amount in the assay gives specific activity.

In your case I would recommend:

I.H. Segel: Biochemical Calculations, 2nd Ed. (Wiley) 1976, ISBN
978-0471774211

Don't be confused by the age of this book, maths hasn't changed much in
the last 30 years!

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