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Peptide in solution

Peptide in solution - Protocols and Methods Forum

Peptide in solution - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 05-08-2009, 09:28 AM
Yvonne Couch
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Default Peptide in solution



Hi all,
Thanks for the brilliant advice and this seems silly now but I don't know
how I would go about lyophilizing a peptide, can anyone help?
Cheers again!
Yvonne

-----Original Message-----
From: Paul J. Phelan [mailto:[Only registered users see links. ]]
Sent: 06 May 2009 19:04
To: Yvonne Couch
Cc: [Only registered users see links. ]
Subject: Re: Peptide in solution

I would concentrate the peptide in a "Speed-vac": you freeze it and
then put it in a centrifuge under vacuum, the solvent will evaporate,
but the PBS salts will be concentrated along with the peptide.
Otherwise, you could lyophilize the peptide and re-dissolve it with
H2O, to whatever volume you like.

Quoting Yvonne Couch <[Only registered users see links. ].ac.uk>:

it




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Old 05-08-2009, 04:19 PM
Nick Theodorakis
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Default Peptide in solution

On May 8, 5:28*am, "Yvonne Couch" <[Only registered users see links. ].ac.uk> wrote:

A lyophilizer is simply a freeze-dryer, which is a vacuum pump and
cold trap hooked up to a chamber in which the samples are placed. A
Speed-Vac is a lyophilizer in which the chamber contains a centrifuge
and is more suitable for small sample volumes. To lyophilze, just
freeze the sample at low temperature (dry ice bath or -80C freezer),
put it in the chamber, and apply the vaccum.

If you are borrowing someone's make sure whatver solvent you are using
is ok with them; some people are fussy about what goes in their cold
trap. You might have better luck if yo need to borrow to find someone
who is doing HPLC since they are probably already drying down samples
that way.

Nick

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