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Yvonne Couch 05-08-2009 09:28 AM

Peptide in solution
 
Hi all,
Thanks for the brilliant advice and this seems silly now but I don't know
how I would go about lyophilizing a peptide, can anyone help?
Cheers again!
Yvonne

-----Original Message-----
From: Paul J. Phelan [mailto:[Only registered and activated users can see links. Click Here To Register...]]
Sent: 06 May 2009 19:04
To: Yvonne Couch
Cc: [Only registered and activated users can see links. Click Here To Register...]
Subject: Re: Peptide in solution

I would concentrate the peptide in a "Speed-vac": you freeze it and
then put it in a centrifuge under vacuum, the solvent will evaporate,
but the PBS salts will be concentrated along with the peptide.
Otherwise, you could lyophilize the peptide and re-dissolve it with
H2O, to whatever volume you like.

Quoting Yvonne Couch <[Only registered and activated users can see links. Click Here To Register...].ac.uk>:

it





Nick Theodorakis 05-08-2009 04:19 PM

Peptide in solution
 
On May 8, 5:28*am, "Yvonne Couch" <[Only registered and activated users can see links. Click Here To Register...].ac.uk> wrote:

A lyophilizer is simply a freeze-dryer, which is a vacuum pump and
cold trap hooked up to a chamber in which the samples are placed. A
Speed-Vac is a lyophilizer in which the chamber contains a centrifuge
and is more suitable for small sample volumes. To lyophilze, just
freeze the sample at low temperature (dry ice bath or -80C freezer),
put it in the chamber, and apply the vaccum.

If you are borrowing someone's make sure whatver solvent you are using
is ok with them; some people are fussy about what goes in their cold
trap. You might have better luck if yo need to borrow to find someone
who is doing HPLC since they are probably already drying down samples
that way.

Nick

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