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Peptide in solution

Peptide in solution - Protocols and Methods Forum

Peptide in solution - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 05-06-2009, 04:35 PM
Yvonne Couch
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Default Peptide in solution



I have a peptide dissolved in PBS at 1mg/ml, I want to concentrate it, is it
possible to get the peptide out of solution in order to concentrate it?

Cheers

Yvonne

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  #2  
Old 05-06-2009, 06:12 PM
Nick Theodorakis
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Default Peptide in solution

On May 6, 12:35*pm, "Yvonne Couch" <[Only registered users see links. ].ac.uk>
wrote:

You could bind it to a (for example) C-18 column, elute it with a
volatile eluant such as acetonitrile, and lyophilize it. There are
some disposable C-18 cartridges called Sep-Pak made by Waters that
might be suitable.

Nick

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  #3  
Old 05-06-2009, 08:28 PM
WS
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Default Peptide in solution

Hi Nick,

will your suggestion also work for larger peptides i.e. proteins? Will
MeCN still work as eluent?
Wo


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  #4  
Old 05-08-2009, 04:12 PM
Nick Theodorakis
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Default Peptide in solution

On May 6, 4:28*pm, WS <[Only registered users see links. ]> wrote:

The best answer I can give is "maybe" or even "probably." If enzymatic
or other biological activity is important to you, it may not be the
best way, though. For a protein, the first thing I would try is to
concentrate by ultrafiltration.

Nick

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  #5  
Old 05-08-2009, 07:31 PM
Dr Engelbert Buxbaum
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Default Peptide in solution

Am 06.05.2009, 12:35 Uhr, schrieb Yvonne Couch
<[Only registered users see links. ].ac.uk>:


Put the solution into a dialysis bag and put some PEG 20,000 outside. The
stuff is hygroscopic and sucks the water out of the bag. Dry Sephadex
works to, but is more expensive. Just look after the thing from time to
time, you don't want all the water removed from your sample.

Centrifugal concentrators (e.g. Millipore) or Amicon pressure cells also
work.
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  #6  
Old 05-09-2009, 03:09 PM
Pipette Filler
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Default Re: Peptide in solution

you could use a kit that works pretty good for that, Proteospin CBED Kit from LabOmics / Norgen
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  #7  
Old 05-14-2009, 05:20 PM
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Default Re: Peptide in solution

You can use the ProteoSpin CBED Kit from Norgen Biotek. That will be usful for this peptide concentration. It will also allow you to elute the peptides in diffrent buffers (Buffer exchange).

Good Luck,

NarNar
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