On Tue, 05 May 2009 00:54:40 +0530 wrote
How to check if the electrodes are OK?
I did soak the membrane in transfer buffer before transfer.
because of milk products in blocking buffer which can be dissolved at 37,I mentioned this because after secondary Ab incubation, 2 of the pretsained markers were missing from the membrane.So, I was wonderin if temp. is the reason for loss of proteins frm. the membrane or wht?
I understand that the pptn. is not because of Ab, but
Is there any such journal? I have many manuscripts to publish then...
Protein science Lab
Dept. of Biological sciences
National University of Singapore