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#1
05-05-2009, 08:28 AM
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 Re: Western trasfer and blotting troubleshooting On Tue, 05 May 2009 00:54:40 +0530 wrote How to check if the electrodes are OK? I did soak the membrane in transfer buffer before transfer. because of milk products in blocking buffer which can be dissolved at 37,I mentioned this because after secondary Ab incubation, 2 of the pretsained markers were missing from the membrane.So, I was wonderin if temp. is the reason for loss of proteins frm. the membrane or wht? I understand that the pptn. is not because of Ab, but Is there any such journal? I have many manuscripts to publish then...  )Shifali Chatrath Graduate Student Protein science Lab Dept. of Biological sciences National University of Singapore Singapore +65-65161210 +65-96393449  |
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#2
05-08-2009, 07:19 PM
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| Re: Western trasfer and blotting troubleshooting Am 05.05.2009, 04:28 Uhr, schrieb shifali chatrath <[Only registered users see links. ]>: eyeball it, if it looks ok, that's sufficient. who hasn't? |
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