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Re: Western trasfer and blotting troubleshooting

Re: Western trasfer and blotting troubleshooting - Protocols and Methods Forum

Re: Western trasfer and blotting troubleshooting - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 05-05-2009, 08:28 AM
shifali chatrath
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Default Re: Western trasfer and blotting troubleshooting





On Tue, 05 May 2009 00:54:40 +0530 wrote
How to check if the electrodes are OK?
I did soak the membrane in transfer buffer before transfer.


because of milk products in blocking buffer which can be dissolved at 37,I mentioned this because after secondary Ab incubation, 2 of the pretsained markers were missing from the membrane.So, I was wonderin if temp. is the reason for loss of proteins frm. the membrane or wht?


I understand that the pptn. is not because of Ab, but
Is there any such journal? I have many manuscripts to publish then... )


Shifali Chatrath
Graduate Student
Protein science Lab
Dept. of Biological sciences
National University of Singapore
Singapore
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+65-96393449
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  #2  
Old 05-08-2009, 07:19 PM
Dr Engelbert Buxbaum
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Default Re: Western trasfer and blotting troubleshooting

Am 05.05.2009, 04:28 Uhr, schrieb shifali chatrath
<[Only registered users see links. ]>:



eyeball it, if it looks ok, that's sufficient.



who hasn't?
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