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RE: Expression and -N end rule

RE: Expression and -N end rule - Protocols and Methods Forum

RE: Expression and -N end rule - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 05-02-2009, 10:52 AM
shifali chatrath
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Default RE: Expression and -N end rule



Yeah! I also tgought so, just wanted second opinion out of text books.
You mean to say If Bulkier groups are present next to Met, MAP will not act and Met will stay with the protein sequence?
I checked the expression with coomassie and silver stain. i cudn't do western because the protein was untagged. Also, I checked in medium (Unconc. n conc.), cell lysates ( sup. n pellet both), I could not find band coressponding to my protein or any overexpressed band on the gel.I analysed abt. 6 recombinant colonies and 2 control colonies as negative ctrl.But nothing. Coomassie was blank in medium, no bands at all.

Thanks
Regards
Shifali

On Wed, 29 Apr 2009 17:55:49 +0530 wrote

Shifali Chatrath
Graduate Student
Protein science Lab
Dept. of Biological sciences
National University of Singapore
Singapore
+65-65161210
+65-96393449
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