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RE: Expression and -N end rule

RE: Expression and -N end rule - Protocols and Methods Forum

RE: Expression and -N end rule - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 04-26-2009, 02:12 PM
David-Paul Minde
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Default RE: Expression and -N end rule



y, and SUMO is a quite small tag. ...
Can also be easlily implemented in basically any (expression) vector easily
via RF cloning (s.attached .. which really works nicely if you have
competent cells as required for good quickchange for just simple
pointmutations as well and use e.g. Phusion as good polymerase) or of course
the other way round if you are not dealing with giants like tition (with
some 33 000 aa which is not very convenient for PCR .
best,
David

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