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bacterias 41(DE3) e C43(DE3)

bacterias 41(DE3) e C43(DE3) - Protocols and Methods Forum

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Old 04-20-2009, 10:11 PM
Itabajara Vaz - UFRGS
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Default bacterias 41(DE3) e C43(DE3)



Hi,

I am needing the C41(DE3), C41(DE3)pLysS and C43(DE3), C43(DE3)pLysS (E.
coli bacterias)
Does anybody have these bacterias?
//Can anybody help me?
Thank you very much,

**C41(DE3), C41(DE3)pLysS and C43(DE3), C43(DE3)pLysS
E. coli BL21(DE3) strains, like Lucigen's E. cloni EXPRESS Competent
Cells provide reliable expression of many proteins cloned into T7
expression vectors (e.g., pET or Lucigen's pSMARTŪ-cDNA vectors).
However, in some cases expression is minimal or not detectable because
the recombinant protein, when expressed, is deleterious or lethal to
these standard BL21 strains. Examples of such toxic proteins include
many membrane proteins, some cytoplasmic proteins, and nucleases.
Unfortunately, successful expression of one or more toxic proteins is
often important to the experimental goal.

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sincerely

--
Itabajara da Silva Vaz Junior
Centro de Biotecnologia - UFRGS
C.P. 15005
Av. Bento Goncalves 9500
Predio 43421 - Campus do Vale
91501-970 Porto Alegre RS Brasil
Fone (51) 33086078 Fax (51)3308-73-09 [00 55 51 3308 7309]
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[Only registered users see links. ]

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