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Cytochrome C western blot

Cytochrome C western blot - Protocols and Methods Forum

Cytochrome C western blot - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 04-12-2009, 05:44 PM
Victor Emelyanov
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Default Cytochrome C western blot



Hi

Using Mab against cyt c (Santa-Cruz, 7H8 clone) in WBA of mitochondrial fractions, I always oserve only band of ca. 70 kDa and never innate protein of 15 kDa. Long ago, I saw the same using Pharmingen Mab. At that time I was said that cyt c forms covalently linked complex with some specific protein and that even relevant paper exists. Did you fix a problem? Indeed covalent complex? Or multimer? Now and then I didn't use chaotropic agent in Laemmli sample buffer (8M urea or Guanidine chloride). Maybe this is important? Don't hesitate to contact me.

Best,

Dr Victor V. Emelyanov, PhD
Senior Research Associate
Marie Curie Fellow

Institute for Cellular
& Molecular Biosciences,
Newcastle University,
Framlington Place,
Newcastle upon Tyne,
NE2 4HH,
United Kingdom




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