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#1
04-04-2009, 04:24 AM
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 Re: Expression of protein * Hi all! I also have expression problem. I tried expressing my protein, cDNA cloned in pET19b in Rosetta gami cells ( beacause it has 5 disulphide bonds and cDNA is from snake)and RIL cells.I couldn't see any expression. Cells were growing normaly.(I hope, the protein is not inhibiting transcription and translation) Protein is 10Kda and was cloned without tag sequence. I mean expected protein should be 10kDa.I confirmed the sequences before cloning and even plasmid isolated from expression hosts. DNA is there but no protein. Any comments??????? On Sat, 04 Apr 2009 DK wrote : Shifali Chatrath Graduate Student Protein science Lab Dept. of Biological sciences National University ofSingapore Singapore +65-65161210 +65-96393449  |
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