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RNA extraction from human adipose tissue

RNA extraction from human adipose tissue - Protocols and Methods Forum

RNA extraction from human adipose tissue - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 04-01-2009, 03:03 PM
larissa yuri
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Default RNA extraction from human adipose tissue



Dear Shelley,
*
I'm a scientist of State University of Campinas (UNICAMP), in Brazil, and our laboratory have researched human isolated adipose tissue and its glucosemetabolism during stress. I very would like to use molecular biology, specificaly the Real-Time PCR technique, to quantify adipocytes enzimes (whole*tissue) and *receptors (isolated adipocytes). But I didn't find a protocol of RNA extraction specific*to adipose tissue. Therefore, I ask you, please, the protocol of RNA Extraction in adipose tissue. I'll write you about my results.
*
Thanks for your attention
*
Larissa Yuri
*
Department of Physiology and Biophysics,
*Institute of Biology (home page: [Only registered users see links. ]),
State University of Campinas (UNICAMP), Campinas, Brazil



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