I am measuring Dopamine release from NGF-differentiated PC12. I am using
HPLC with C18 reverse phase column in 10 % methanol isocratic elution.
It seem that after 3-4 times the column get clotted, the pressure go high
and we need to wash a lot with metanol (much more than regular
wash). Sometimes the injection needle is blocked and it takes time to
Is anyone knows a better way to wash the column after meausiring dopamine
from PC12 supernatant?
In my lab. some people measure norepinephrine from synaptosomes and after
measurment HPLC works well without problems.