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vector ligation problem

vector ligation problem - Protocols and Methods Forum

vector ligation problem - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 02-26-2009, 02:32 PM
huazhongw
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Default vector ligation problem




Dear memebers,
I am working with vector constuction. I inserted a fragment into a vector by routine single-digest \electrophoresis\recovery\ligation\tranformation method. When I recut recombinant plasmids with the same enzyme, I run into a problem that the vector fragment size become smaller than the original cut vector.
I don't know why and need help!


Huazhong Wang


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