I don't advise you to lyophylize antibodies! Mabs is very sensitive proteins ( like a woman) for this harsh procedure. A great loss of activity is observed in many cases. Alternative methods for Mab storage are:
solubilize Mab at high concentration (5-10 mg\ml important
) in 10mM NaPO4 150mM NaCl 10 mM borate pH 8.0 + 20% glycerine important
, make aliqotes ( keep in mind that one repeated freezing - loss 15-20% Mab activity) and keep frozen at -20C.
Another exotic method is:
Keep Mabs frozen under argon at -80C.
If you've purified antibodies and decide to use protein during one - two weeks, you can add L-arginine ( up to 0.5M) in storage solution for protein stabilization in aqueous solution and 0.02% NaN3. Of course, for cell culture experiments you must dialyze protein to remove NaN3 and probably arginine , otherwise cells can't understand you