Difficult pcr templates

Phusion polymerase & 5x GC buffer altogether (from Finnzymes) works wonders....
good luck

P.


-----Original Message-----
From: [Only registered users see links. ] on behalf of Scott Brown
Sent: Tue 20/01/2009 04:04
To: [Only registered users see links. ]
Subject: Difficult pcr templates

Hi,

I have recently been using clontechs GC-2 advantage pcr mix to aid in
amplification of GC rich sequences, can anyone recommend or has anyone
trialed any other pcr mixes on the market?

Scott

Scott Brown
PhD Candidate
Molecular Carcinogenesis Program
Children's Cancer Institute of Australia for Medical Research
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AUSTRALIA

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Children's Cancer Institute Australia for Medical Research is the only independent medical research institute in Australia devoted to research into the causes, prevention, better treatment and ultimately a cure of childhood cancer. Our vision is to save the lives of all children with cancer and to eliminate their suffering.

The information contained in this message and any annexure is confidential and intended only for the named recipient(s). If you have received this message in error please contact the sender immediately and destroy the original message.

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In article <[Only registered users see links. ].net >, "Carmen P Pena Diaz" <[Only registered users see links. ].ac.uk> wrote:

Or just use PfuUltra Fusion II from Stratagene with its stock
buffer and 4% DMSO... Totally fool-proof.

DK