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Difficult pcr templates

Difficult pcr templates - Protocols and Methods Forum

Difficult pcr templates - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 01-20-2009, 04:04 AM
Scott Brown
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Default Difficult pcr templates



Hi,

I have recently been using clontechs GC-2 advantage pcr mix to aid in
amplification of GC rich sequences, can anyone recommend or has anyone
trialed any other pcr mixes on the market?

Scott

Scott Brown
PhD Candidate
Molecular Carcinogenesis Program
Children's Cancer Institute of Australia for Medical Research
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Children's Cancer Institute Australia for Medical Research is the only independent medical research institute in Australia devoted to research into the causes, prevention, better treatment and ultimately a cure of childhood cancer. Our vision is to save the lives of all children with cancer and to eliminate their suffering.

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  #2  
Old 01-20-2009, 09:06 AM
chovek69
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Default Difficult pcr templates

On Jan 20, 6:04*am, "Scott Brown" <[Only registered users see links. ].edu.au> wrote:
See this... helps alot with difficult templates...

[Only registered users see links. ]



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  #3  
Old 01-20-2009, 11:01 AM
StewJW
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Default Difficult pcr templates

Thanks for the previous post, interesting paper. A possible commercial
solution, as well a the PCRX Enhancer System mentioned in the paper,
is AccuPrime™ GC-Rich DNA Polymerase, I've no idea why this polymerase
should work any better:
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  #4  
Old 01-20-2009, 12:31 PM
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Default Re: Difficult pcr templates

Hi Scott,
try DMSO 1-5% for tough PCR templates especially GC rich ones
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