TBS/Casein 1% freeze

amicia · #1 (**permalink**) 10-11-2006, 01:21 PM

Hi all,
I'm doing WB with TBS/casein 1%, I would like to know if somebody freeze TBS/casein with the primary antibody in order to reuse it.
Greetings and thank you to the reply

kiki06 · #2 (**permalink**) 10-11-2006, 04:49 PM

Hey Amicia!

Yes you can freeze your 1' primary antibody with TBST in the -20 C

I have done this and the antibody is ok for a while (ie a few months)

amicia · #3 (**permalink**) 10-12-2006, 10:10 AM

Hi Kiki,
many thanks for the answer, did you try to re-use many time or only twice? I mean heat up and freeze it again.

kiki06 · #4 (**permalink**) 10-12-2006, 11:34 PM

Yes the antibody can work for a few freeze-thaws (2-4 sometimes more!), unless its an antibody for a phospho-protein (in the case of a phosphorylation antibody, I would use it 1 more time or use fresh).

cheers

amicia · #5 (**permalink**) 10-13-2006, 09:52 AM

Thank you Kiki.

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