Gewtting the concentration of PCR product and cloning
In <[Only registered users see links. ].net >,
shifali chatrath <[Only registered users see links. ]> wrote:
The problem is with your comprehension of English. Let me clarify:
If one wrote, "PCR products .... ARE A-tailed", then it would indicate
that products made with a proof-reading polymerase are already A-tailed.
On the other hand, if one writes "PCR products .... MUST BE A-tailed", then
it indicates that the products made with a proof-reading polymerase are
NOT A-tailed, and therefore MUST BE A-tailed for them to be cloned into a
Hope that helps you .....
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