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Gewtting the concentration of PCR product and cloning

Gewtting the concentration of PCR product and cloning - Protocols and Methods Forum

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  #1  
Old 12-28-2008, 05:31 AM
Aawara Chowdhury
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Default Gewtting the concentration of PCR product and cloning



In <[Only registered users see links. ].net >,
shifali chatrath <[Only registered users see links. ]> wrote:


The problem is with your comprehension of English. Let me clarify:

If one wrote, "PCR products .... ARE A-tailed", then it would indicate
that products made with a proof-reading polymerase are already A-tailed.

On the other hand, if one writes "PCR products .... MUST BE A-tailed", then
it indicates that the products made with a proof-reading polymerase are
NOT A-tailed, and therefore MUST BE A-tailed for them to be cloned into a
TA-vector.

Hope that helps you .....

AC
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  #2  
Old 12-28-2008, 08:38 PM
Pow Joshi
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Default Fwd: Gewtting the concentration of PCR product and cloning

---------- Forwarded message ----------
From: Pow Joshi <[Only registered users see links. ]>
Date: 2008/12/28
Subject: Re: Gewtting the concentration of PCR product and cloning
To: [Only registered users see links. ]



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  #3  
Old 12-29-2008, 12:49 AM
David-Paul Minde
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Default Gewtting the concentration of PCR product and cloning

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