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#1
12-25-2008, 04:15 PM
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 Gewtting the concentration of PCR product and cloning Hi all members, I have amplified PCR product of 1250 bp from Genomic DNA my question is 1 Whether it can be cloned in TA cloning vector the required size. 2. how to calculate the concentration of the PCR product after the reaction  |
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#2
12-25-2008, 05:35 PM
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| Gewtting the concentration of PCR product and cloning In article <[Only registered users see links. ]>, Shri <[Only registered users see links. ]> wrote: Yes, no problem. If you used proofreading polymerase, it would have to be A-tailed first. If Taq and the like, no need to. Most MW standards come with know concentrations. Run a bit of your PCR reaction with serial dilution of the standards and estimate the amount of your product from a ~ 1 kbp band. Or gel-purify your product and do A260/A280 on it. DK |
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| cloning , concentration , gewtting , pcr , product |
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