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Gewtting the concentration of PCR product and cloning

Gewtting the concentration of PCR product and cloning - Protocols and Methods Forum

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Old 12-25-2008, 05:15 PM
Shri
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Default Gewtting the concentration of PCR product and cloning



Hi all members,
I have amplified PCR product of 1250 bp from Genomic DNA
my question is
1 Whether it can be cloned in TA cloning vector the required size.
2. how to calculate the concentration of the PCR product after the
reaction
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Old 12-25-2008, 06:35 PM
DK
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Default Gewtting the concentration of PCR product and cloning

In article <[Only registered users see links. ]>, Shri <[Only registered users see links. ]> wrote:

Yes, no problem. If you used proofreading polymerase, it
would have to be A-tailed first. If Taq and the like, no need to.


Most MW standards come with know concentrations. Run a bit
of your PCR reaction with serial dilution of the standards and
estimate the amount of your product from a ~ 1 kbp band.
Or gel-purify your product and do A260/A280 on it.

DK
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