a follow up question regarding dissolving H2O insoluable compounds inDMSO

Hi

I saw the interesting discussion of dissolving PP1 in PBS.

I always take it for granted that one can dissolve water insoluble stuff inan organic solvent then add it to a water based solution. But I have neverthought about it. Why is that possible? Why it wont precipitate out from the solution? That should still depends on the compound's solubility in water. Does it mean the organic solution is simply for a high concentration stock?

BTW, is there a database for drug solubility?

have a nice Xmas

SZ

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Subject: Methods Digest, Vol 43, Issue 16



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Today's Topics:

1. Re: An inquiry (Dr. Hiranya S. Roychowdhury)
2. RNAi (Jayanta Tarafdar)
3. methylcellulose colony question (Scott Brown)
4. RACE sequencing problem (Scott Brown)


----------------------------------------------------------------------

Message: 1
Date: Fri, 19 Dec 2008 10:29:33 -0700 (MST)
From: "Dr. Hiranya S. Roychowdhury" <[Only registered users see links. ]>
Subject: Re: An inquiry
To: "Shahrzad Jalali" <[Only registered users see links. ]>
Cc: [Only registered users see links. ]
Message-ID: <[Only registered users see links. ] su.edu>
Content-Type: text/plain;charset=iso-8859-1

I don't believe you can expect a solution of PP1 in PBS. It will form a
colloidal suspension. A colloidal suspension will also elicit immune
response. Remember that the peritonial fluid is also polar and the PP1
will come out of solution there too. DMSO, however, does the rat very
little damage in microliter quantities intra-peritonially. I believe the
PBS is used to minimize any shock. PP1 is soluble also in ethanol, and it
may be a better alternative solvent for the injection. Just a thought!




--
Hiranya S. Roychowdhury, Ph.D.
Asst. Professor,
Health & Public Services
Dona Ana Community College
New Mexico State University
Las Cruces, NM 88003



------------------------------

Message: 2
Date: Fri, 19 Dec 2008 23:23:03 +0530
From: "Jayanta Tarafdar" <[Only registered users see links. ]>
Subject: RNAi
To: [Only registered users see links. ]
Message-ID:
<be8bf3be0812190953i3c458dfm296893a5f4357ec0@mail. gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Dear Subscribers
Methods Digest

We have been working on the backcross breeding of rice with transgenic
rice
carrying RNAi gene against virus. Would anybody help me regarding use of
non-radioactive probe for analysis of the transcript.
I would appreciate if anybody can give short-cut method in this regard
and
urgently waiting for the answer.
Jayanta
India


------------------------------

Message: 3
Date: Sat, 20 Dec 2008 04:33:48 +1100
From: "Scott Brown" <[Only registered users see links. ].edu.au>
Subject: methylcellulose colony question
To: <[Only registered users see links. ].indiana.edu>
Message-ID:
<2A67EA781EC7F949A2AB0A0D07A86C6A02972BC5@mail01.c cia.local>
Content-Type: text/plain; charset="iso-8859-1"

Hi,

I have been growing Nalm-6 colonies in methocult for about a year now, the ratio of media is 1.6ml methocult, 1.2ml IMDM and 1.2ml FCS with or withoutdrug depending on what i am doing.
Has anyone had any experience with maintaining the methocult for at least 3weeks, frequently my methocult looks as though is has dessicated, i have water in the incubator and i also plate 2 wells with PBS to ensure humidity....but...it still happens, any ideas or suggestions?

S

Scott Brown
PhD Candidate


Children's Cancer Institute Australia for Medical Research is the only independent medical research institute in Australia devoted to research into the causes, prevention, better treatment and ultimately a cure of childhood cancer. Our vision is to save the lives of all children with cancer and to eliminate their suffering.

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------------------------------

Message: 4
Date: Sat, 20 Dec 2008 04:38:40 +1100
From: "Scott Brown" <[Only registered users see links. ].edu.au>
Subject: RACE sequencing problem
To: <[Only registered users see links. ].indiana.edu>
Message-ID:
<2A67EA781EC7F949A2AB0A0D07A86C6A02972BC6@mail01.c cia.local>
Content-Type: text/plain; charset="iso-8859-1"

Hi,

I have been sequencing constructs about 1kb in size that i have obtained from a second race reaction, i have pruified the product, spec'd it and ensured when run on a gel i get a band.
This has worked for a while, however the last 1 or so constructs i have hadsequenced have failed, the chromatogram looks as though the primer has noteven bound to the template, it is simply noise that drops off to an almostundetectable signal.
Can anyone make any suggestions as to what I should be looking at to troubleshoot this? I sent my positive control as well as 1 sample from my next set of rave reactions to be sequenced, both were successful, then when i sentthe remaining 8, all 8 failed.
Might i need to redesign my primer i am using for sequencing? Any other thoughts?

S

Scott Brown
PhD Candidate


Children's Cancer Institute Australia for Medical Research is the only independent medical research institute in Australia devoted to research into the causes, prevention, better treatment and ultimately a cure of childhood cancer. Our vision is to save the lives of all children with cancer and to eliminate their suffering.

The information contained in this message and any annexure is confidential and intended only for the named recipient(s). If you have received this message in error please contact the sender immediately and destroy the original message.



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