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How primer concentration can me measured?

How primer concentration can me measured? - Protocols and Methods Forum

How primer concentration can me measured? - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 12-16-2008, 01:17 PM
Kamalian, Laleh
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Default How primer concentration can me measured?



Hi. I usually use NanoDrop to double check the concentration written on the
tube anyway. Only make sure you use the specific OD on the tube for the primer.
Best of luck.
L.

-----Original Message-----
From: [Only registered users see links. ]
[mailto:[Only registered users see links. ].indiana.edu] On Behalf Of TRAN HO QUANG
Sent: 15 December 2008 16:09
To: [Only registered users see links. ]
Subject: How primer concentration can me measured?

Does anyone tell me how can measure primer concentration (working solution)

I am facing problems: the PCR reactions are not consistent. It is suggested that
primer concentrations are not exact as it is 10 uM (working solution). The
questions is can i use agarose or NanoDrop to check primer concentration?.



Any suggestions are warmly welcome!

Digifellow




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