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PAGE gel sticked on both plates

PAGE gel sticked on both plates - Protocols and Methods Forum

PAGE gel sticked on both plates - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 12-08-2008, 03:41 PM
Patricia C Arbour-Reily
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Default PAGE gel sticked on both plates



1) I have had other brands of "Sigmacote" go bad......2)Rain-X , for
your car windows , works OK but you must treat more often...its a bit
more green and will do in a pinch while you replace Sigmacote , if gone
bad. 3) We have had plates get gunk on them from something in the wash
up...one time, a student ran all plates thru a dishwasher and used the
correct soap for a dishwasher but the wrong soap for the plates ,
obviously...... I don't know how that was corrected
Pat


-----Original Message-----
From: [Only registered users see links. ]
[mailto:[Only registered users see links. ].indiana.edu] On Behalf Of Nicosnake
Sent: Sunday, December 07, 2008 8:44 PM
To: [Only registered users see links. ]
Subject: PAGE gel sticked on both plates


HI everyone, i've been having this problem whit my acrylamide gels:
after
acrylamide is polymerized between the two glass plates it's really hard
to
separate both plates and after a long effort i can take them appart only
to
realize that the gel has sticked to BOTH plates, the gel is actually cut
in
half. I treat surfaces of both plates, one with bind silane (so the
acrylamide sticks to this one) and the other with Sigmacote to prevent
the
gel from sticking. But still the gel sticks to both surfaces and after i
separate the glases it's useless. Has anyonehad this kind of trouble??
ANy
suggestion is accepted.
--
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