microbial load

[aftabac]

Hi all!

what is the best way to determine bacterial load in lab? and also please guide about the operational limits of the microbes in animal cell culture lab.

hope to get some good response


best regards
aftab

[kalaig]

You mean bacteria in the air? The best way is to filter air through a presterilized membrane filter with 0.2 micron membrane and filter the air in the different points of the lab (esp. under the laminar flow!). Put the membrane on a general bacterial (nutrient agar) as well as fungal medium (Malt extract medium or rosebengal agar). If you know the flow rate of the vacuum pump, then you can work the actual air sucked in based on the time of operating the vacuum pump from which bacterial/ fungal load per cubic meter of air can be calculated.

[moleculardude]

Excellent post Kalaig!


There are also some HEPA filters, very expensive although they work extremely well.

[kalaig]

Thanks! MD!;-)