Lipid Extraction

[JoanaB]

Dear all,

My name is Joana, and I am currently starting to work with lipids.
I use Drosophila as a model system.

Since I am new to this field, I am having some difficulties in getting enough good quality lipid extracts in my samples.

Does anyone knows a protocol suited for experiments using Drosophila tissue?

Most protocols I see use the Folch or Bling Dyer Methods.
In these protocols, after concentration of the sample (by speed vac), in what solvent do you ressuspend your lipids? (I will be quantifying the lipids using colorimetric methods, so if I can avoid chloroform or methanol, that would be excelent, since the 96-well plates I have are not resistant to ant of these solvents).
Also, does anyone knows if this "concentration" step be done just by "air-drying" (I do not have a speed vac)?

Thanks,
Joana
Reply With Quote