| | Re: Pierce North2South Direct HRP Lebelling and detection system
The North2South Pierce kit that both labels and detects (Cat#17175), which is really a combination of two other kits, is $999 (Australian).
If I were to buy the two kits from them separately, it would be Cat#17095: $754 and Cat#17075: $564. So its cheaper buying it all together. But a grand for a labelling and detection system just sounds rather expensive to me. Its a real pity that they discontinued their Direct HRP Labeling and detection system a few years back, i think that would have really been what i was looking for.
So I started to look around, so far ive found the Roche Applied Science
DIG Labeling and Detection Starter Kit. I havent really properly read up about it yet and at this present moment cant recall the difference between kit I and kit II. Its probably because i really havent read the manuals on either..yet.
The Kit I (Cat#7145832910) goes for $559.
and Kit II (Cat#11585614910) goes for $567.
I wont be needing to make mg of RNA, but I will need to make ug of mRNA, which I will be using in the Clontech cDNA substraction kit, which going by the publications seems to be the way to go if youre performing a cDNA subtraction hybridisation. The thing is that this method can give a high level of false positives, so I need to use a dot blot type of assay to weed out the false positives. This is where the Pierce detection systems come in, to allow labelling and detecting a set of different probes, to verify positives as true ones.