hi i'm new here. soorrry if i make any mistake.
i am using Bradford Assay to quantify protein and BSA as my standard.
1) I prepared a master mix of 2mg/mL of BSA and diluted them from 0.1mg/mL to 1.2mg/mL in a linear range.
2) thereafter i diluted the bradford reagent at 2:7, adding 4mL of reagent to 14mL of ddH2O.
3) I added 20uL of each BSA standard into 980uL of the diluted bradford reagent, prepared in step 2.
4) samples were incubated for 15 minutes and OD595 were gathered to plot a curve.
Problem: all values i obtained is lower than 0.03. what do u think the problem is?
UV curvette and GV curvette. what's the difference? which should i use.
however i also use nanodrop, the value still remain as BAD
thanks alot in advance =)