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running ssDNA (30-1000nt) in denatured conditions

running ssDNA (30-1000nt) in denatured conditions - Protocols and Methods Forum

running ssDNA (30-1000nt) in denatured conditions - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 11-04-2008, 07:51 PM
TC
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Default running ssDNA (30-1000nt) in denatured conditions



Hi all,

I am trying to run DNA under denatured conditions. Has anyone run single
stranded DNA (30nt to 1000nt) under denatured conditions.

I am trying to decide whether to run a low percentage urea acrylamide gel or
an alkaline agarose gel. Any thoughts/ideas? I am thinking maybe running a
low percentage (3-4%) urea acrylamide gel, but wonder that it may not be
able to resolve ssDNA above couple hundred nucleotides in length even in low
acrylamide such as 3-4%? Any thoughts? Has anyone run alkaline agaorse for
ssDNA 30nt-1000nt? If so, may I ask for your conditions?

THANKS!

Tracy
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  #2  
Old 11-19-2008, 08:10 AM
cutest_chemist@yahoo.com
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Default running ssDNA (30-1000nt) in denatured conditions

On Nov 5, 12:51*am, TC <[Only registered users see links. ]> wrote:

Hi...
I m currently using 20% Polyacrylamide gel(PAGE)for ssDNA (20-30 nt)
and 15% PAGE for ssDNA of 30-50mer.Its giving good results...Hope this
will help u..For more lengthy strans i think u should try 8-10% PAGE.

Regards
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