Dynabeads for epitope-tagged protein immuno-precipitation

So I am giving the Protein G dynabeads a try for IPs of myc-tagged
proteins from mammalian cell lysates. The information that comes with
the system if not detailed and there is little technical information
to be found on invitrogen's web site.

Does anyone routinely use this system for protein
immunoprecipitation? I've only tried the 'direct' method so far, but
I have not been successful. I've only tried the basic protocol
provided, so if someone has a protocol to share, that would be great!

Thanks,
Jonathan

[Roald]

Hi Jonathan

I see this was posted a while ago. How are you getting on with the Dynabeads Protein G?
I have been using these beads for yonks and am really happy with their performance in IP. I'm more than happy to share my experience with you. Let me know if you're still having problems. :-)
Roald

[dgorasia]

Jonathan,

I'm planning to do IP with Myc tagged protein and Dynabeads. If it's worked with you then can you please give me the protocol.

Thanks,
Dhana

PS: which Myc antibody do you use?