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Protein elution from PVDF membrane

Protein elution from PVDF membrane - Protocols and Methods Forum

Protein elution from PVDF membrane - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 10-24-2008, 02:40 AM
jh
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Default Protein elution from PVDF membrane



maybe high salt solution will help.

Jianhao

2008/10/23 Amit <[Only registered users see links. ].in>:

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Old 10-25-2008, 10:37 PM
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Default Re: Protein elution from PVDF membrane

This is my best guess:
cut the spot or band off the membrane, place it in a epi tube.
Add 0.1%TFA/40% Acetonitrile solution in PBS.
Key ingredient is the Acetonitrile.
Incubate 10 minutes or more then remove the membrane, protein should be in solution.
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