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A question about pET system

A question about pET system - Protocols and Methods Forum

A question about pET system - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 10-23-2008, 08:17 PM
Qingwu Shen
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Default A question about pET system



Hi everybody,

I have a question about pET system. As you know, pET vectors do not have lacZ -
peptide and are not applicable to blue/white screening. However, why the colonies
still become blue on plate when XL1 blue transformed with pET28 basic vector is
grown for more than 36 hours? Thank you very much.

shen

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