Disease causing protein

[Xeonophon]

hello everyone, i need some help with a problem i need to solve.
I suspect that a disease is caused by the under expresion of a protein, how would i go about proving this? How would i be able to create this protien using recombination to turn it into a treatment for that disease?
Any help will be great, can figure it out.

[danfive]

Originally Posted by Xeonophon

hello everyone, i need some help with a problem i need to solve.
I suspect that a disease is caused by the under expresion of a protein, how would i go about proving this? How would i be able to create this protien using recombination to turn it into a treatment for that disease?
Any help will be great, can figure it out.

First, you can exacerbate the condition by knocking out gene expression entirely, or isolate the diseased tissue (like in cell culture) reproduce the condition and "treat it" with extra protein.
Secondly, you would isolate the gene, splice it into a vector and tranform a host, then induce the host to produce your protein from the vector.

[padma]

hi,
there are some disease caused by prions.Prion can cause disease & can infect the host.prions are same as virus but having no nuclic acid.mad cow disease is an example of this type in which a protein can modify the protein further this altered protein can cause the disease.