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How can I concentrate my protein?

How can I concentrate my protein? - Protocols and Methods Forum

How can I concentrate my protein? - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 09-28-2007, 07:05 AM
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Smile How can I concentrate my protein?



Hi all,

I ran a Western blot recently and I know I did not load enough protein (I loaded only 0.8 micrograms even though I had crude protein) because I did not have enough. Not surprisingly, I didn't get any bands for my control. Does anyone know how I can concentrate my protein?

Thanks in advance!
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Old 09-28-2007, 04:30 PM
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Default Re: How can I concentrate my protein?

Microcon or Ultrafree MC filter columns (Millipore), TCA:Acetone precipitation, Use less lysis/extraction buffer volumes.
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Old 11-04-2007, 01:10 AM
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Default Re: How can I concentrate my protein?

you can try some precipitation method, like TCA etc
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Old 11-13-2007, 09:30 PM
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Default Re: How can I concentrate my protein?

u can pass ur sample in a speed vac and than resuspend it directly in the sample buffer.
good luck
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