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09-27-2007, 06:19 PM
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 Masking effect in ELISA I am 'making' my first ELISA (ran only one ELISA before in my life and it was a kit). I'm trying to make sure I don't have a masking effect. When I diulte my plasma, my absorbance values increase but only by .2-fold for every 10-fold dilution. Is this a great enough absorbance increase with dilution for me to be concerned about MASKING? Also since I am looking for a protein,  the usual methods of fixing a masking effect - heating or decreasing pH probably are not a good idea. Does anyone have other suggestions?Thanks, LIZAELISA        |
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Re: Masking effect in ELISA 
Linear Mode
