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| Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique. |
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07-26-2006, 03:08 PM
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 Gene synthesis Hi is it possible that instead of whole gene synthesis from some company we ask them to synthesis oligonucleotide of about 50bp in length of that particular gene and then we join them in our lab by DNA ligase to synthesize our required gene. waiting for some good answer aftab        |
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08-01-2006, 03:05 AM
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 Re: Gene synthesis Hi I synthsize 150 base pair of sequence from geneart a german company and they clone it in to vector i guess pGMT so you can try if you want it work perfectly for me |
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08-03-2006, 04:29 AM
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| Re: Gene synthesis HI i need the whole gene including the protein coding region and the strong promoter region, so i ask once again that is it possible that if we have a set of oligonucleotide of about 50bp in size and want to construct a gene of 600bp then is it possible to join the oligonucleotide and construct the gene instead of synthesizing the whole gene. regards aftab |
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