A sales rep. from Millipore just left the lab and almost convinced me
to buy what he calls "a revolution in your western-blot experience": a
simple device (SNAP i.d.) that makes all incubations shorter by
forcing all solutions "through" the membrane (using vacuum). [Only registered users see links. ]
It certainly looks like a much simpler, quicker and cleaner way to do
all incubation for the westerns, but I would like to hear from people
who have actually used the mechine. The question is: will the 850
euros investment increase our happiness?
In <[Only registered users see links. ].net> ,
TR <[Only registered users see links. ]> wrote:
We've been testing it for the last 3 weeks. So far, it has proven to
not work as well doing our incubations in little plastic bags.
The background is very low (we use 0.125% non-fat milk with the SNAP i.d.,
and 5% non-fat milk for blots done the old fashioned way), however, the
sensitivity is much better the old fashioned way (about 10 - 50 times
Email: echo 36434455860060025978157675027927670979097959886449 930P | dc
On Oct 16, 1:36*am, Kyle Legate <[Only registered users see links. ]> wrote:
The demo unit we tried out for one afternoon (not much time, but
that's what they gave us) it worked as well for most antibodies and
even better for some. Since we've gotten our new unit, I've not had
any success and I am sticking to my old method until I have more time
to troubleshoot it.
Yes, this will make your life much easier! It will take a little time to figure out the antibody concentrations for your primary antibody. I use the Snap ID almost all of the time. Some times it will not work with some primary antibodies but I have had a 100% success with all my secondary antibodies that I have used. Additionally it can be incorporated into your traditional WB protocols.
Washing steps and blocking steps can be done using the Snap. This is a huge time saver if you have 2 or more blots. I have also determined you can reuse the cassettes but you need to take care of them.
I think this thing is the best thing to happen to WB in a long time!!!