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#1
09-26-2008, 11:11 AM
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 for estimation of acid phosphatase hi, Im satheesh kumar, i plan to estimate the acid phosphatase activity in coffea. so anybody wil send me some our good suggestion to me...  |
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#2
09-26-2008, 09:07 PM
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| for estimation of acid phosphatase Hi, cheapest way should be pNPP (p-nitrophenyl phosphate) as substrate. If you prefer higher sensitivity and/or prefer fluorescent detection, you might choose MUPF (methyl umbelliferyl phosphate) or ELF phosphate, just to mention some possibilities. If you want to use chemiluminescence, check CSPD/CPD-star substrates. pH is adjusted with appropriate buffer in all cases. Best reagrds, Wo On Sep 26, 1:11*pm, "satheesh kumar" <[Only registered users see links. ]> wrote: |
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| acid , estimation , phosphatase |
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