Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protocols and Methods Forum
Register Search Today's Posts Mark Forums Read

Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


Transfection myoblast

Transfection myoblast - Protocols and Methods Forum

Transfection myoblast - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 09-24-2008, 01:55 PM
laure d'astarac
Guest
 
Posts: n/a
Default Transfection myoblast



Hi,

I have to start transfection of AON and siRNA in my lab, where nobody has
experience...

So several questions about myoblast transfection with small RNAs (or
AONs...)

1/ what is the best transfection method to use: Electroporation Vs
Lipfectamin Vs other?

2/ If someone has general rules about minimum and maximum amount of AON -Si
RNA to use in transfection, in order to be efficient but not toxic... I
suppose i have to do several quantities, but values are arround ng,
microg???

3/ When harrest cells after transfection: 24h? 48? test both?

I am sorry about these basic questions.... and thank you for your help....

--
Claire Navarro
Stem cell laboratory
University of Milan - Ospedale Maggiore Policlino di Milano -
Padiglione Ponti, II piano
Via F. Sforza, 35
20122 Milan
Italy
Tel: 00 39 02 55 03 38 52
00 39 02 55 03 38 74
Reply With Quote
Reply

Tags
myoblast , transfection


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
HepG2 Cell Transfection TMU Transfection Forum 2 10-05-2009 02:38 PM
Any Transfection kit for HepG2 cells? drkashyap Transfection Forum 5 02-25-2009 11:10 PM
Cell Transfection moleculardude Cell Biology and Cell Culture 4 04-18-2008 05:22 PM
Cell Adhesion Problem After Transfection gandalfthegrey Cell Biology and Cell Culture 1 04-09-2008 02:40 PM
how do you improve transfection efficiency? really puzzled! bb Protocols and Methods Forum 1 07-23-2005 04:12 PM


All times are GMT. The time now is 05:02 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.10955 seconds with 16 queries