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Removal of Melanin from DNA/RNA

Removal of Melanin from DNA/RNA - Protocols and Methods Forum

Removal of Melanin from DNA/RNA - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 09-18-2008, 01:31 PM
Selvam-Akumulla Philomina
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Default Removal of Melanin from DNA/RNA



Respected Sir

I am Mrs.Selvam working in Dermatology Skin Cancer Research.
Our major projects are Melanoma and Lymphoma. I want to try
GITC to remove melanin from biopsie, later extract DNA for
HLA Typing.

Can you help me right recepie of GITC, I have the following
recepie for GITC: 5.0M Guanidine Isothiocyanate
8% Mercap-toethanol
10mM EDTA
50mM Tris
pH 7.5
weather it is right one or not. attachment of protokol.

Thanks in advance

With Regards
Philomina Selvam




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