Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protocols and Methods Forum
Register Search Today's Posts Mark Forums Read

Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


info abt linker in GFP fusion protein

info abt linker in GFP fusion protein - Protocols and Methods Forum

info abt linker in GFP fusion protein - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 09-16-2008, 03:27 PM
Itisam Sarangi
Guest
 
Posts: n/a
Default info abt linker in GFP fusion protein



Hi all,
I am new to the cell biology field. I am presently trying to tag eGFP to my
protein of intrest in Pmyc vector. I am trying to prepare a vector with MCS
before and after eGFP.

what shd be the minimum length of linker so tht I can see GFP in cell after
transfection?
which one is better C -terminal or N terminal tagged?
What if I give MCS before or after GFP so tht gfp remains in frame still I
can put any other protein? where can I find regarding MCS

any help in this matter will be helpful.


Thanking you

--
Itisam Sarangi
Reply With Quote
Reply

Tags
abt , fusion , gfp , info , linker , protein


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Human Cytome Project - Update 24 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 1 08-01-2010 02:18 PM
Human Cytome Project - Update 6 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 0 01-06-2005 11:18 AM
New Saccharomyces Sequences 11/27/04 Mike Cherry Yeast Forum 0 11-29-2004 12:39 AM
New Saccharomyces Sequences 05/19/04 SGD Sequences Yeast Forum 0 05-23-2004 04:06 PM
New Saccharomyces Sequences 09/10/03 SGD Sequences Yeast Forum 0 09-11-2003 01:33 AM


All times are GMT. The time now is 05:08 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.15315 seconds with 16 queries