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pET15b (Novagen) IPTG induction

pET15b (Novagen) IPTG induction - Protocols and Methods Forum

pET15b (Novagen) IPTG induction - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 09-08-2008, 11:00 AM
E. Lis
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Default pET15b (Novagen) IPTG induction



It is possible that your protein is present in inclusion bodies, and
lysozyme and sonication will not disrupt those. Have you tried lysis by
8M urea?
You could also try running entire bacterial cells suspended in SDS-PAGE
sample buffer (as 10 min in sample buffer at 100^C should do the trick).

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