| |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique. |
 |
| | LinkBack | Thread Tools | Display Modes |
|
#1
09-08-2008, 11:00 AM
| |||
| |||
 pET15b (Novagen) IPTG induction It is possible that your protein is present in inclusion bodies, and lysozyme and sonication will not disrupt those. Have you tried lysis by 8M urea? You could also try running entire bacterial cells suspended in SDS-PAGE sample buffer (as 10 min in sample buffer at 100^C should do the trick).  |
|
| Tags |
| induction , iptg , novagen , pet15b |
| Thread Tools | |
| Display Modes | |
|
|
Similar Threads | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| Problems with IPTG induction of PT7/lac Operon | Pipetti13 | Recombinant Protein Forum | 0 | 07-29-2009 07:10 PM |
| IPTG induction | aftabac | Microbiology Forum | 5 | 03-05-2009 01:47 AM |
| pET15b (Novagen) IPTG induction | Paul J. Phelan | Protocols and Methods Forum | 2 | 09-17-2008 08:55 PM |
| pET15b (Novagen) IPTG induction | Ho-Leung Ng | Protocols and Methods Forum | 0 | 09-05-2008 06:34 PM |
| IPTG induction in non E.coli strains | Francesca | Protocols and Methods Forum | 22 | 03-24-2007 12:17 AM |
Linear Mode
