pET15b (Novagen) IPTG induction
It is possible that your protein is present in inclusion bodies, and
lysozyme and sonication will not disrupt those. Have you tried lysis by
You could also try running entire bacterial cells suspended in SDS-PAGE
sample buffer (as 10 min in sample buffer at 100^C should do the trick).
|All times are GMT. The time now is 05:58 AM.|
Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2015, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved