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-   -   pET15b (Novagen) IPTG induction (http://www.molecularstation.com/forum/protocols-methods-forum/20067-pet15b-novagen-iptg-induction.html)

E. Lis 09-08-2008 11:00 AM

pET15b (Novagen) IPTG induction
 
It is possible that your protein is present in inclusion bodies, and
lysozyme and sonication will not disrupt those. Have you tried lysis by
8M urea?
You could also try running entire bacterial cells suspended in SDS-PAGE
sample buffer (as 10 min in sample buffer at 100^C should do the trick).



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